The determination of all monitored substances and their metabolites and consequently their influence on the representation of biologically active substances and nutritional composition of production requires the inclusion of modern instrumental analytical techniques with high-quality chemometric assurance of analytical data.
As part of this activity, they are being developed and / or developed. modified, analytical methods for determination of anthropogenic organic substances and their possible metabolites in various matrices (soil, water, plant, animal, food) by gas and liquid chromatography combined with high resolution mass detection (GC-QTOF, LC-QTOF, MALDI imaging).
Similarly, changes in metabolism of treated plants are determined with a focus on nutritionally, toxicologically and ecologically important substances. For the determination of organic compounds of elements (As, Se, Hg) is used a combination of HPLC with inductively coupled plasma mass spectrometry (HPLC-ICPMS), which is a robust and very sensitive technique enabling the identification and quantification of a wide spectrum of inorganic and organic elements of elements.
Furthermore, positively acting biologically active substances and their transfer in individual stages of the food chain and volatile substances in different stages of the food chain by GC-MS are defined.
These can serve as markers of positive or negative changes in the product - plant, animal, food.
The total carbohydrate, fat and protein content of the product is also determined - plant, animal, food - and individual components of these groups - carbohydrates, fatty acids, triacylglycerols, sterols, proteins (GC-MS, LC / MS, NMR, UV / VIS / IR spectroscopy, 2D PAGE, MALDI-TOF), depending on the application of the investigated anthropogenic substances.
